Clodronate is very effective in the treatment of hypercalcemia and cancer metastases to bones. It can significantly reduce the development of new metastases, as well as to decrease the risk of bone fractures, which is very high in such cases. Besides, it may also reduce the pain associated with this disease. Clodronate liposome, also known as clodrosome, seems to be the best way of delivering this compound to tissues.
Liposomes are lipid-based nanoparticles. Liposomal encapsulated drugs are mostly used in academic research laboratories. Some new anti-cancer compounds such as this one require a liposome delivery system to be closed in vivo. Target organs for this particular compound are kidneys and bones.
Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.
If you get in any type of direct contact with it, make sure to follow the procedure. If it get in contact with your eyes, you should flush them with clean water for some time repeatedly, approximately fifteen minutes or so. If it get in touch with your skin, wash it with water and soap. If you inhale it, go outside on fresh air immediately. For more serious exposures, maybe you should have a respirator or even oxygen nearby. If you swallow it, rinse your mouth and call a doctor.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When liposomes encapsulated clodronate gets to tissue, macrophage will ingest and digest it, but they will only destroy this liposome capsule, not the active component. It will be released within this tissue, and then accumulated. Macrophage depleted tissue will allow performing different in vivo researches related to macrophages.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
The suspension should be kept at 4 degrees of Celsius, up to one month. The important thing to remember is that it should never be frozen, or exposed to temperatures over 30 degrees. It is also important to wait until this suspension reach room temperature before injecting it to your animals. Other ways your results could be different than expected.
It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.
Liposomes are lipid-based nanoparticles. Liposomal encapsulated drugs are mostly used in academic research laboratories. Some new anti-cancer compounds such as this one require a liposome delivery system to be closed in vivo. Target organs for this particular compound are kidneys and bones.
Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.
If you get in any type of direct contact with it, make sure to follow the procedure. If it get in contact with your eyes, you should flush them with clean water for some time repeatedly, approximately fifteen minutes or so. If it get in touch with your skin, wash it with water and soap. If you inhale it, go outside on fresh air immediately. For more serious exposures, maybe you should have a respirator or even oxygen nearby. If you swallow it, rinse your mouth and call a doctor.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When liposomes encapsulated clodronate gets to tissue, macrophage will ingest and digest it, but they will only destroy this liposome capsule, not the active component. It will be released within this tissue, and then accumulated. Macrophage depleted tissue will allow performing different in vivo researches related to macrophages.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
The suspension should be kept at 4 degrees of Celsius, up to one month. The important thing to remember is that it should never be frozen, or exposed to temperatures over 30 degrees. It is also important to wait until this suspension reach room temperature before injecting it to your animals. Other ways your results could be different than expected.
It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.
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